|
KMID : 1225720150070020190
|
|
Allergy, Asthma & Immunology Research : AAIR
2015 Volume.7 No. 2 p.190 ~ p.194
|
|
|
Eosinophils Modulate CD4+ T Cell Responses via High Mobility Group Box-1 in the Pathogenesis of Asthma
|
|
Shim Eun-Jin
Chun Eun-Young
Lee Hyun-Seung
Bang Bo-Ram
Cho Sang-Heon
Min Kyung-Up
Park Heung-Woo
|
|
|
Abstract
|
|
|
|
Eosinophils have been reported to modulate T cell responses. Previously, we reported that high-mobility group box 1 protein (HMGB1) played a key role in the pathogenesis of asthma. This study was conducted to test our hypothesis that eosinophils could modulate T cell responses via HMGB1 in the pathogenesis of asthma characterized by eosinophilic airway inflammation. We performed in vitro experiments using eosinophils, dendritic cells (DCs), and CD4+ T cells obtained from a murine model of asthma. The supernatant of the eosinophil culture was found to significantly increase the levels of interleukin (IL)-4 and IL-5 in the supernatant of CD4+ T cells co-cultured with DCs. HMGB1 levels increased in the supernatant of the eosinophil culture stimulated with IL-5. Anti-HMGB1 antibodies significantly attenuated increases of IL-4 and IL-5 levels in the supernatant of CD4+ T cells co-cultured with DCs that were induced by the supernatant of the eosinophil culture. In addition, anti-HMGB1 antibodies significantly attenuated the expressions of activation markers (CD44 and CD69) on CD4+ T cells. Our data suggest that eosinophils modulate CD4+ T cell responses via HMGB1 in the pathogenesis of asthma.
|
|
|
KEYWORD
|
|
|
Asthma, eosinophil, high-mobility group box 1 protein (HMGB1), T cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|